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| Article Name : | | | ISOLA TION, BIOCHEMICALCHARACTERIZA TION AND IDENTIFICA TION OFPHOSPHA TE SOLUBILIZING PSEUDOMONAS FROM RHIZOSPHERE OFSOYBEAN AND STUDYOFITS ANTIMICROBIAL ACTIVITY | | Author Name : | | | R. N. JADHA V | | Publisher : | | | Ashok Yakkaldevi | | Article Series No. : | | | GRT-2228 | | Article URL : | |  | Author Profile View PDF In browser | | Abstract : | | | Rhizosphere is the region where soil & roots makes contact. The microbial growth is enhanced by nutritional substances released fr om plant tissues. It is highly favorable habitat for proliferation and metabolism of numerous microbial types. Next to nitrogen phosphorus is most important plant nutrient. Microorganisms influence phosphorus availability to plant through the process of mineralization & immobilization. Pseudomonas species live in a commensal relationship with plants. Pseudomonas species have ability to produce different types of metabolites like antibiotics which are harmful to plant pathogens. This practice is known as bio-control. In the present work screening of phosphate solubilizing Pseudomonas fr om rhizosphere of soybean was done by using selective media. Further study was done on Pikovaskys medium. After incubation at room temperatur e for 4-6 days clear zone (Zone of solubilizzation) were observed around the colony . The quantification of phosphorus solubilizing ability has been evaluated as PSE. Based on morphological, physiological & biochemical tests, Pseudomonas species were identified. It was observed that two isolates of Pseudomonas PSPF-02 & PSPF -05 shows high PSE. The isolates of Pseudomonas were further tested for antimicrobial activity . It was found that Bacillus subtilis NCIM2010; E .coli NCIM2064; Klebsiella pneumoniae NCIM 2719 were highly sensitive while C. albicans NCIM3557; Fusarium oxysporium (soil isolate) Xanthomonas compestris NCIM2956 were less sensitive to the isolates of Pseudomonas. | | Keywords : | | |
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