| The present study was aimed to evaluate the antioxidant and anticancer activity of the marine sponge Hyatella cribriformis associated bacteria Bacillus cereus SBS02 isolated from Gulf of Manner, Tamilnadu, India. Hyatella cribriformis containing bacterial extracts were tested for Hep-2 cell line. The percentage viability of the cell line was carried out by using colorimetric method. The cytotoxicity of the partially purified protein sample 70 kDa and 8kDa on Hep-2 cell was evaluated by MTT assay. Both the protein sample has significant cytotoxic effect on Hep-2 cell line in the concentration range between 3.906 μl to 1000 μl using MTT assay. R2 value of protein 70 kDa was 0.997 and for 8 kDa was 0.964. The above proteins were relatively evaluated for the radial scavenging activity using DPPH. The compound concentration was increased from 2 to 10 mg/ml, the DPPH scavenging rate of 70 kDa protein was 25%, 40%, 65%, 80% and 95.4% and similarly 8 kDa was 25.3%, 48.69%, 84.7% and 96.1% respectively. |