ISSN No: 2231-5063
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Article Details ::
Article Name :
Calcium Impact On Cadmium Induced Alterations In Selected Oxidative Stress Enzymes In The Fresh Water Teleost, Oreochromis Mossambicus (Tilapia)
Author Name :
Obaiah Jamakala And A. Usha Rani
Publisher :
Ashok Yakkaldevi
Article Series No. :
GRT-2960
Article URL :
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Abstract :
Cadmium (Cd) is an important xenobiotic, non biodegradable cumulative pollutant in aquatic ecosystems. The present study was carried out to know the impact of calcium (Ca) on Cd induced oxidative stress enzyme alterations in the selected tissues of fresh water fish, Oreochromis mossambicus (Tilapia). After acclimatization, fish were exposed to sublethal concentration of Cd (1/10th of LC50/48h, i.e., 5ppm) for 7, 15 and 30 days (d) period. Then 15d Cd exposed fish were subjected to Ca supplementation (1ppm) for 7, 15 and 30d time intervals. After specific exposure periods tissues like gill, kidney and brain were isolated and used for Cd for the assay of glutathione-S- transferase (GST), glutathione peroxidase (GPx) and estimation of lipid peroxidation (LPO) levels in both control as well as experimental fish. Significant elevation in LPO levels were observed during Cd exposure and maximum elevation of LPO was found in 30d Cd exposed gill tissue (16.074±0.089 μ moles of MDA formed / gm wet wt. of the tissue). Further the activity levels of GST and GPx were significantly inhibited under Cd stress. However with Ca supplementation there was significant reduction in LPO levels and elevation in the activity levels of the GST and GPx. Maximum activity levels of GST and GPx were observed in 30d Ca supplemented fish kidney. Our findings elucidate the beneficial role of Ca as vital supplement in reducing the Cd burden in the fresh water fish, Oreochromis mossambicus (Tilapia).
Keywords :
  • science education,
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