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| Article Name : | | | PRODUCTION, PURIFICATION AND CHARACTERIZATION OF TANNASE FROM NATIVE ASPERGILLUS SP USING SYZYGIUM CUMINI (L) SKEELS (EUGENIA JAMBOLANA) SEED POWDER | | Author Name : | | | N. Shanmugapriya , S. Ramganesh , Nitin Labhane | | Publisher : | | | Ashok Yakkaldevi | | Article Series No. : | | | GRT-3521 | | Article URL : | |  | Author Profile View PDF In browser | | Abstract : | | | Tannin acyl hydrolase (E.C.3.1.1.20) commonly referred as tannase, is an industrially important enzyme that is mainly used in the food, chemical, beverage and pharmaceutical industry. In this study, tannase yielding native fungal isolate were identified as Aspergillus niger and Aspergillus flavus. The fungal culture produced high yields of extracellular tannase (30.05±1.32 U/ml and 23.43±1.43 U/ml) under solid-state fermentation (SSF) using Syzygium cumini seed powder. To enhance the production level of the enzyme, different culture conditions were optimized with the native isolates and observed that 30°C incubation temperature, pH 6.0, 1%(w/v) tannic acid as carbon source and 1%(w/v) Ammonium nitrate as a nitrogen source for maximum tannase activity. Tannase was purified from both isolates showed 2.29 & 1.43 purification fold with a specific activity of 10.24mg/ml & 12.36 mg/ml protein. This is the first report on production of tannase by A. niger & A.flavus, giving a higher yield of enzyme under SSF with Jamun seed powder as the substrate. | | Keywords : | | |
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